DiBAC4(5) 膜电位荧光探针

【温馨提示】：见我司整理的膜电位荧光探针产品专题。

产品简介：

慢速响应探针（Slow-response probes）包括阳离子碳花菁、罗丹明和阴离子氧鎓醇，都适用于检测非兴奋细胞因呼吸活性、离子通道渗透性、药物结合和其他因素引起的平均膜电位变化。

DiBAC4(5)(Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol)是带阴离子的慢速响应电位敏感探针，在pH4.5~7.5的范围内表现出非pH敏感的荧光特征（最大激发和发射波长分别为590nm和616nm）。能够进入去极化细胞，之后结合到胞内蛋白或膜上。一旦结合，DiBAC4(5)的荧光特性加强，往红光区迁移。增加的细胞去极化引起增多的染料内流和增高的荧光信号。DiBAC4(5)的负电荷阻止其进入线粒体，因此，适用于细胞膜电位测定。

产品特性：

1） CAS NO：63560-89-4

2） 同义名：Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol

3） 分子式：C29H42N4O6 

4） 分子量：542.67g/mol

5） Ex/Em：590/616 nm

6） 溶解性：溶于DMSO

7）化学结构图：

保存条件: 

-20℃干燥避光保存，有效期至少一年。

产品使用：（来自文献，仅供参考）

实验方法（膜电位染色）：DiBAC4(5) is a translational MP dyes that redistributes within the cell membrane as MP changes. MP depolarization results in an increase in fluorescence intensity of cells at Ex/Em = 590/616nm. Conversely, hyperpolarization is indicated by a decrease in fluorescence. PMA-differentiated THP-1 cells were cultured in 35mm imaging dish with a concentration of 4x105cells/ml. Cells were stained in 200 nM DiBAC4(5)in 5mM K+ buffer at 37°C for 30 min. Then the staining buffer was removed without washing and crystals (silica, Al(OH)3, CPPD) or inhibitors (glyburide or CFTRinh-172) in fresh 5mM K+buffer containing 200nM DiBAC4(5) was added to the cells. For high K+ condition, staining buffer was replaced with 100mM K+ buffer containing 200nM DiBAC4(5). We captured 5 different fields at each time-point on fluorescent microscope, and analyzed the fluorescence intensity of all the cells by ImageJ.

实验结果（膜电位染色）：

Fig. Depolarization or Hyperpolarization of Membrane Potential May Affect the Activation of NLRP3 Inflammasome.THP-1 cells were stained with DiBAC4(5) to check the effect of CFTRinh-172 on MP. Representative images and relative fluorescence unit (RFU) plotting are shown. Each point was mean ± SEM of cells from at least 12 fields in 2 independent experiments. Scale bar, 20 μm.

注意事项：

1.      本品用有机溶剂（比如：DMSO）配制的储存液，请根据单次用量分装，置于≤-20℃避光保存。

2.      荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

3.      为了您的安全和健康，请穿实验服并戴一次性手套操作。

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