德国MN NucleoBond Xtra Midi 转染级质粒中提试剂盒
货号:740410.50
规格:50 preps
品牌:德国MN
2nd generation of anion exchanger for fast purification of plasmid DNA
Selling unit | 10 Prep(s), 50 Prep(s), 100 Prep(s) |
Application | Transfection-grade plasmid DNA isolation |
Target | Plasmid DNA |
CE certified | No, research use only |
Technology | Anion exchange chromatography |
Brand | NucleoBond |
Format | Midi prep |
Handling | Gravity flow |
Lysate clarification | Column filter |
Automated use | Yes, can be automated on the Andrew+ the pipetting robot |
Sample material | Bacteria, E. coli |
Sample amount | < 200 mL (high copy), < 400 mL (low copy) |
Vector size | < 300 kbp |
Typical yield | 500 µg |
Theoretical binding capacity | 800 µg |
Typical purity A260/A280 | 1.8−1.95 |
Endotoxin level | 1−10 EU/µg DNA |
Isopropanol precipitation | Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi |
Preparation time | 70 min/prep |
Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
Storage temperature | 15−25 °C |
Shelf life (from production) | 39 Month(s) |
Hazardous material | Yes |
中提大提产品比较:
NucleoBond(R) XtraMidi | NucleoBond(R) Xtra Maxi | |||
技术 | 阴离子交换色谱法 | |||
产品形式 | Midi gravity-flow columns | Maxi gravity-flow columns | ||
裂解液澄清 | 柱过滤器 | 柱过滤器 | ||
样品材料 | < 200 mL (高拷贝) < 400 mL (低拷贝) | < 600 mL (高拷贝) < 1 200 mL (低拷贝) | ||
载体大小 | < 300 kbp | < 300 kbp | ||
产量 | 250 μg | 1000 μg | ||
A260/A280 | 1.80 – 1.95 | 1.80 – 1.95 | ||
异丙醇沉淀 | Xtra Midi | Xtra Midi Plus | XtraMaxi | XtraMaxi Plus |
Centrifugation | NucleoBond(R) Finalizer | Centrifugation | NucleoBond(R)Finalizer Large | |
制备时间 | 70min/preps | 30min/preps | 80min/preps | 35min/preps |